Dataset supporting "Inhibitory feedback from the motor circuit gates mechanosensory processing in Caenorhabditis elegans"

This is the dataset used in the PLOS Biology publication titled "Inhibitory feedback from the motor circuit gates mechanosensory processing in Caenorhabditis elegans" by S. Kumar, A. K. Sharma, A. Tran, A. M. Leifer. The preprint of this work can be accessed at: https://doi.org/10.48550/arXiv.2301.02709

Previously published closed-loop high throughput optogenetic delivery system (Liu, Mochi, et al. "A high-throughput method to deliver targeted optogenetic stimulation to moving C. elegans populations." PLoS biology 20.1 (2022): e3001524.) was used to track worms crawling on an agarose plate as well as deliver optogenetic stimulus to worms in real time depending on the current behavior state of the worms. In this work, worms were optogenetically stimulated in various behavior states such as forward, turn, and reverse. We investigated key reversal neurons such as AIZ, RIM, AIB, AVE, and AVA as well as the turning neurons RIV, SMB, and SAA.

Datasets are arranged in folders with the corresponding figure name. For e.g. the folder 'figure_2_and_supplementary_figure_S4' contains the dataset used in generating figure 2 and supplementary figure S4. Each folder consists of .mat file for a worm strain and the corresponding behavior assay. For e.g. in 'figure_2_and_supplementary_figure_S4' folder, there is a .mat file named as 'raw_data_AIB_closed_loop_blue_stim.mat'. This file contains the data for optogenetic stimulation in a closed-loop behavior assay for the strain expressing an excitatory opsin in neuron AIB. The .mat file has the following information on each worm track: worm's velocity, centerline, ellipse ratio, optogenetic stimulus, etc. More details about each of the experiments carried out in this study can be found in Table 2 of the PLOS Biology manuscript.

The MATLAB code used to analyze the data and generate figures presented in this work is available on GitHub at https://github.com/leiferlab/kumar-sensorimotor-integration.git

If you use this dataset, please cite the following works in addition to citing the dataset itself:

1. Liu, Mochi, et al. "A high-throughput method to deliver targeted optogenetic stimulation to moving C. elegans populations." PLoS biology 20.1 (2022): e3001524.
2. Kumar, Sandeep, et al. " publication titled "Inhibitory feedback from the motor circuit gates mechanosensory processing in Caenorhabditis elegans." PLoS biology (2023).