SUMMARY STATISTICS
1. overall
• number of datasets = 1
• number of donors = 1
• original number of cells = 1654
2. barcode fate
┌──────────┬────────────────────────────┐
│barcodes │ why deleted │
├──────────┼────────────────────────────┤
│ 173 │ failed UMI filter │
│ 54 │ failed IMPROPER filter │
│ 25 │ failed FOURSIE_KILL filter│
│ 11 │ failed QUAL filter │
│ 8 │ failed GRAPH_FILTER filter│
│ 5 │ failed WHITEF filter │
│ 4 │ failed WEAK_CHAINS filter │
│ 1 │ failed DOUBLET filter │
│ 281 │ total │
└──────────┴────────────────────────────┘
3. for the selected clonotypes
┌────────┬────────────────────────┬──────────────────┬───────┐
│chains │ clonotypes with this │ cells in these │ %│
│ │ number of chains │ clonotypes │ │
├────────┼────────────────────────┼──────────────────┼───────┤
│1 │ 139 │ 140 │ 10.2│
│2 │ 295 │ 959 │ 69.8│
│3 │ 16 │ 262 │ 19.1│
│4 │ 4 │ 12 │ 0.9│
│total │ 454 │ 1373 │ 100.0│
└────────┴────────────────────────┴──────────────────┴───────┘
• number of clonotypes having at least two cells = 144
• number of intradonor cell-cell merges = 919
• number of intradonor cell-cell merges (quadratic) = 25,915
• number of cells having 1 chain = 218
• number of cells having 2 or 3 chains = 1152
• estimated B-B doublet rate = 0.3% = 3/942 = cells with 4 chains / cells with 2 or 4 chains
• mean over middle third of contig UMI counts (heavy chain) = 331.32
• mean over middle third of contig UMI counts (light chain) = 1860.48
• mean over middle third of cell UMI counts for cells having two chains = 3141.60
• mean UMIs per cell = 5845.16
• mean UMIs per cell having two chains = 6276.57
• for reads contributing to UMIs in reported chains, mean reads per UMI = 6.25
GLOBAL VARIABLES
d_inconsistent_% = 2.28
d_inconsistent_n = 747